lentiviral Search ResultsScientist with extensive experience in driving complex research initiatives and delivering impactful results. Renowned for fostering collaborative env... View More
Top Skills ⦁ Next-Generation Sequencing (NGS): Library preparation, quality analysis, sequencing across Illumina platforms, protocol modification, optimization, and troubleshooting.⦁ DNA/RNA Engineering: Expertise in recombinant DNA techniques, DNA construct engineering, and engineering of RNA molecules.⦁ In Vitro Transcription: Skilled in in vitro transcription of mRNA with various nucleotide modifications for enhanced efficacy, including encapsulation into lipid nanoparticles for gentle cellular delivery.⦁ Nucleic Acid Extraction: Experienced in RNA/DNA extraction from FFPE samples, cells, and tissues.⦁ PCR Techniques: Proficient in PCR, qPCR, RT-PCR, and ddPCR.⦁ Transfection Methods: Expertise in lipid-based and electroporation transfection techniques.⦁ CRISPR Technology: Skilled in CRISPR/Cas gene editing and generating edited cell lines.⦁ Tumor Biology: Expertise in tumor processing, primary cell line generation, maintenance, and characterization.⦁ Flow Cytometry: Proficient in multicolor flowcytometry using Cytek Aurora.⦁ Immune Assays: Expertise in Elispot (functional T cell assays), Mesoscale Discovery, and Luminex platforms.⦁ Imaging Systems: Skilled in using eSight (Agilent) and Incucyte (Sartorius) for live-cell imaging and analysis.⦁ Molecular Assays: RNA functionality testing, immunohistochemistry, and immunofluorescence techniques.⦁ Lentiviral Production: Expertise in producing and optimizing lentiviral vectors for gene delivery.⦁ Analytical Chemistry: Proficient in HPLC, Triple Quadrapole Mass Spectrometry, Radioimmunoassay (RIA), and infrared imaging for protein analysis.⦁ Protein/Enzyme Assays: Experience with protein interaction assays using Dyna beads, Luciferase assays, and enzyme kinase assays.⦁ Protein Analysis: Expertise in infrared imaging, chemiluminescence detection, spectrophotometry, HPLC, mass spectrometry, and RIA.⦁ Tissue Culture: Skilled in maintaining and modifying patient-derived T-cells and other cell lines.⦁ Data Analysis: Proficient in data analysis using Excel, Prism, Accuri, and HTG Reveal software.⦁ Animal Techniques⦁ Rat Sleep Studies: Experienced in performing surgeries for electrode and microdialysis probe placement, with sleep data analysis using TestPoint software.⦁ Murine Models: Expertise in oral gavage treatment and xenograft murine models, including tumor measurement and treatment evaluation Certifications A W A R D S & C E R T I F I C A T I O N S⦁ Professional Excellence Program Professional Excellence Program-Issued by CHOP · Jul 2017Issued by CHOP · Jul 2017, Associated with Children's Hospital of PhiladelphiaBest Poster Award Best Poster Award⦁ Issued by The Children's Hospital of Philadelphia · Feb 2016Issued by The Children's Hospital of Philadelphia · Feb 2016, Associated with The Children's Hospital of Philadelphia⦁ Group 1: Biomedical Researchers Group 1: Biomedical Researchers CITI Program Issued Sep 2022 · Expires Sep 2025Issued Sep 2022 · Expires Sep 2025 Credential ID 51422287 https://www.citiprogram.org/verify/?w7d17ab67-94e4-4a2a-8f4e-eb75e1ac0858-5142228 Show More
Highly motivated, hard-working, and diligent stem cell biologist with 10+ years of experience in stem cell research and over 4+ years of experience in... View More
Top Skills HPSC/iPSC culture: hPSC culture (antibiotic-free), characterization (for pluripotency), maintenance (feeder-free and xeno-free), passaging (manual and enzymatic), cryopreservation, transfection using lentiviral, lipofectamine (lipid-based transfection) and electroporation, cell bank creation and distribution, iPSC reprogramming Differentiation: tri lineage differentiation methods using EBs, spin EBs and monolayer method, vascular, cardiac and mesodermal differentiation, beating cardiac EBs from knock-in hPSCs. Gene editing: CRISPR/Cas9 RNA design and delivery, eGFP and RFP fusion-based reporter line generation, lentiviral, retroviral mediated construct delivery Molecular Biology techniques: DNA, RNA, Protein isolation, PCR, qPCR, Western blot, ELISA Immunofluorescence, IHC, Paraffin sectioning, Flow Cytometry and FACS, Imaging and confocal microscopy, Bioprinting Animal studies: Animal handling, colony maintenance, Genotyping, Breeding strategies, IP and subcutaneous Injection, anaesthetic machine handling, transplantation, Splenic injection, mouse surgical studies, animal irradiation, mouse dissection, liver cells isolation and perfusion, bone marrow cells isolation, necropsy, drug administration, nude and SCID mice handling and maintenance, generation of irradiated mouse embryonic fibroblasts (MEFs) as feeders. Show More
Ph.D. Molecular biologist with 12+ years of research experience specializing in gene therapy, viral vector biology, and quantitative analytical method... View More
Top Skills Cell Biology & Assays: Mammalian and primary cell culture, stable cell line development transfection (lipid/electroporation), stable cell line development, assay development & optimization, lentiviral/AAV transduction & titration Molecular Biology: CRISPR/Cas9 gene editing, RT-qPCR, RNA/DNA extraction, cloning, siRNA knockdown, ELISA, Western blot, proteomics (SILAC, LC-MS/MS) Imaging & Cytometry: Confocal microscopy, ICC/IHC, flow cytometry In Vivo: Mouse models of bone marrow failure & retinal disease — handling, dosing, tissue collection & processing Bioinformatics & Data: GraphPad Prism, ImageJ, basic data analysis & visualization Show More
Detail-oriented researcher in cancer research and precision oncology, skilled in biotechnology, molecular biology, and immunology. Experienced in desi... View More
Top Skills PCR QPCR Gel Electrophoresis Western Blotting Northern Blotting Show More
Biomedical researcher with over six years of specialized experience in stem cell biology and CAR-T cell therapy, demonstrating a strong track record o... View More
Top Skills Mammalian cell culture Lentiviral gene delivery Gene editing: CRISPR and BE Flow cytometry and cell sorting Cell-based assays: CTV, cytokine secretion, killing assay and restimulation assay Certifications Certificate on Mycotechnology, Royal Bio Research Centre, 05/01/14 Show More
Oncology scientist with expertise in immuno-oncology research, tumour microenvironment biology and biologics-focused drug discovery. Experienced in de... View More
Top Skills 3D tumour microenvironment models Immune/tumour co-culture assays T-cell proliferation and cytotoxicity assays Multiparameter flow cytometry Confocal microscopy Show More
Translational oncology Ph.D. candidate with extensive training in cancer cell biology, cancer metabolism, photodynamic therapy (PDT), redox biology, l... View More
Top Skills Cancer Biology and Therapeutics: photodynamic therapy, therapy-response assays, treatment resistance models, apoptosis/cell-death analysis, combination therapy testing, HNSCC models, breast cancer models. Molecular and Cellular Biology: mammalian cell culture, primary cell culture, qPCR/RT-PCR, western blotting, immunocytochemistry, ELISA, siRNA/shRNA, CRISPR-Cas9, plasmid and lentiviral transfection, cloning, flow cytometry, comet assay, wound-healing assay, cell-cycle analysis. Metabolism and Biomarkers: metabolite extraction, LC-MS-based metabolite analysis, glutathione/redox metabolism, NADPH/NADP-related metabolic analysis, oxidative stress pathway interpretation. In Vivo Oncology: mouse handling, tumor implantation, intravenous drug administration, oral gavage, in vivo fluorescence imaging, ex vivo biodistribution imaging, tumor response analysis, tissue collection. Imaging and Analysis: live-cell fluorescence imaging, two-photon microscopy, confocal microscopy, NIR imaging, organelle colocalization, ImageJ, Leica LAS X, Maven, FCS Express, Adobe Photoshop. Show More
Motivated and skilled Scientist with a strong foundation in cancer biology. Supported by an MRes in Cancer and hands-on industry experience in antibod... View More
Top Skills Lab Techniques: Mammalian Cell Culture, Flow Cytometry (FACS), ELISA, CRISPR, Lentiviral Transduction. Analytical Skills: Experimental Design & Analysis, Strategic Planning, Biostatistics (Prism, FlowJo, Minitab). Antibody Techniques: Antibody-Drug Conjugates, Immunoassays, Cytotoxicity Assays. Soft Skills: Scientific Communication, Public Speaking, Cross-functional Collaboration, H&S Compliance. Show More
Senior Scientist with 10+ years of experience in cellular and biochemical assay development, targeted protein degradation, high-throughput screening, ... View More
Top Skills Targeted Protein Degradation: HiBiT degradation assay, HaloProtac3 assay, NanoBRET ternary complex formation, TR-FRET, AlphaLISA, NanoBiT, CETSA, Target engagement assays, Cell permeability assays, HiBiT blotting, E3 ligase deconvolution (BioID, Co-IP/MS), In vitro and in vivo ubiquitination assays High-Throughput Screening & Automation: 1536-well HTS miniaturization, HighRes BioSolutions integrated automation (Cellario software), Mosquito LV liquid handler, TR-FRET and fluorophore-based biochemical HTS, Assay QC (Z', S/B, EC50), TIBCO Spotfire data analysis and visualization Cell-Based & Biochemical Assays: Show More
An independent multidisciplinary scientist with over 8 years of experience in clinical microbiology, cancer biology, and scientific writer.
Excel... View More
Top Skills Cell culture :Anaerobic and aerobic bacterial culturing, Mammalian cell culture, bacterial co-culture, and invasion assays, microscopic examination of bacteria and cancer cell lines co-cultures, designing plasmid for lentiviral transduction. Molecular Biology :Western blotting, ELISA, Luminex multiplex assay, qPCR, RT-PCR, DNA- RNA extraction, molecular cloning, primer and construct design, protein expression, and isolation from bacterial cells Cell-based assays :Proliferation assays, cell fractionation, cell migration-invasion assay, cell imaging, 3D confocal microscopy, live-cell microscopy (IncuCyte), PACT Clarity technique, neutrophil migration assay, Histology tissue sections techniques Analytical Chemistry Nuclear magnetic resonance, Gas chromatography, High-Performance Liquid chromatography, Thin layer chromatography, Mass Spectrometry, Proteomics, Transcriptomics Software : Statistical analysis by GraphPad Prism, ImageJ, Image Studio, SnapGene, PymoL, ChimeraX, Imaris , imageJ Show More
