knockdown Search ResultsDedicated heavy machinery operator with extensive experience at Mountain States Contractors Inc. Skilled in operating various rollers and excavators, ... View More
Top Skills Heavy Machine Operator, Knockdown Roller, Pneumatic Roller, Finish Roller, Laborer, Shovel, Rake, Climb Power Tools, Jack Hammer, Pogo Stick, Plate Compactor Pipe Layer, Top Man, Water Truck, Bilingual, English/Spanish Certifications Certified osha10: EZ EXCAVATING , MSI - 2018/2025 Show More
Experienced research professional with a strong academic background from the University of Chicago, specializing in immune-oncology. Proficient in mol... View More
Top Skills Molecular Biology: Genome-wide CRISPR screen and NGS analysis Generating knockouts, knockdowns (stable or inducible), overexpression vectors Proficient in basic molecular biology techniques: qPCR, PCR, Western blot, DNA/RNA isolation, scRNA-sequence Cell Culture: Creating knockout or knockdown cell lines (stable or inducible) Culturing human or mouse primary cells and cell lines Handling mouse ES cells Lentivirus transduction Car-T cell culture Primary T cell culture and stimulation Recombinant protein expression in cell line Generating reporter cell lines Immunology: Flow cytometry analysis ELLISPOT assay ELISA (enzyme-linked immunosorbent assay) Cell sorting techniques Tumor Models: Designing, developing, and validating therapeutic candidates in tumor models Generating tumor immunology mouse models Designing and creating knock-in and knock-out mouse models Conducting tumor preclinical experiments using GEM (genetically engineered mouse) tumor models and transplantation models Different dose of drug administration in tumor models (IP, IV, Intratumoral) Other Skills: Designing lipid synthesis experiments and performing mass spectrometry analysis Immunofluorescence (IF) staining of human tissues and cell lines Confocal imaging Ferroptosis detection assays Drug screening using reporter cell lines Writing and publishing scientific articles Presenting data in meetings Excellent communication skills Show More
Dedicated researcher with extensive experience in cancer biology, biotechnology, and molecular biology. Skilled in mammalian cell culture, gene expres... View More
Top Skills Xenograft and PDX models Ip/iv/sc injections Blood withdrawal Primary cell isolation Gene expression Show More
As someone who has lived across three culturally rich countries (India, the United States, and South Korea) for over 25 years combined, I have develop... View More
Top Skills International salesInternational Sales & Client Management: Experience negotiating with global partners (Europe, India, CIS) Overseas Market Development: Conducted international exhibitions, sourced and secured new clients SKD (Semi-Knockdown) Project Coordination: End-to-end support for manufacturing partnerships (esp. in India) Video Conferencing & Remote Sales: Comfortable conducting high-level discussions virtually across time zones Language & Communication SkillsTrilingual Fluency: Korean (native), English (native/professional), Hindi (fluent) Certifications TOEIC Driver License 2nd Class Ordinary Driver License 2nd Class Small Show More
Experienced in stem cell biology, hematology, and immunology, with a strong track record in leading complex research projects and teams. Skilled in pr... View More
Top Skills Stem Cell Biology: Isolation of human cord blood, mouse bone marrow, and mobilized peripheral blood HSCs and progenitors, HSCs ex vivo expansion and culture; colony formation assays, cell cycle, apoptosis, and proliferation assays. Immunology: Flow Cytometry, Luminex, and ELISA assays. T cells, B cells, and dendritic cells isolation, culture, and phenotyping. Animal Models: IACUC protocols writing and submission, Immuno-oncology subcutaneous and orthotopic humanized mouse models, HSCs transplantation and mobilization co-genic mouse models. Mouse injections (i.v., s.c., i.p, oral gavage, stereotaxic). High Throughput Sequencing: Single cell sequencing, and cell hashing, Spatial Muti-Omics (Nanostring-GeoMx-DSP), Next-Generation Sequencing (NGS) library preparation. Histology and Imaging: Leica BondRx - IHC/IF, QuPath. Show More
Experienced with conducting thorough research and analyzing data to support academic projects. Utilizes strong organizational skills and attention to ... View More
Top Skills DNA/RNA Libraries Preparation Cell Culture DNA/RNA/Protein Extraction qPCR Western Blot Show More
Dedicated Certified Journeyman Carpenter with expertise in metal framing, drywall installation, and mentoring. Committed to enhancing project efficien... View More
Top Skills Specialized in all gauges of metal framing (12 gauge to 25 gauge). Installed all types of metal and wood frame doorbucks including knockdown and solid frame. Installed all forms of drywall including densglass and plywood including T-111, durock and coreboard installation including framing. Specialized in wood and metal protection on the job site. Specialized in all forms of acoustical ceilings including drywall. Show More
Ph.D. Molecular biologist with 12+ years of research experience specializing in gene therapy, viral vector biology, and quantitative analytical method... View More
Top Skills Cell Biology & Assays: Mammalian and primary cell culture, stable cell line development transfection (lipid/electroporation), stable cell line development, assay development & optimization, lentiviral/AAV transduction & titration Molecular Biology: CRISPR/Cas9 gene editing, RT-qPCR, RNA/DNA extraction, cloning, siRNA knockdown, ELISA, Western blot, proteomics (SILAC, LC-MS/MS) Imaging & Cytometry: Confocal microscopy, ICC/IHC, flow cytometry In Vivo: Mouse models of bone marrow failure & retinal disease — handling, dosing, tissue collection & processing Bioinformatics & Data: GraphPad Prism, ImageJ, basic data analysis & visualization Show More
A cancer researcher with expertise in molecular and cellular biology, non-invasive imaging, and preclinical cancer models involved in drug discovery a... View More
Top Skills Cell Biology Assays and Techniques: Mammalian Cell Culture (Monolayer, Spheroid and Organoid) and Cell Based Assays (Cell Viability, Apoptosis, Clonogenicity, Migration and Invasion Assays), Drug Screening, Co-Culture, T Cell Isolation, Flow Cytometry, Microcopy and Fluorescent Microscopy Molecular Biology Techniques: DNA Extraction, RNA Extraction, Protein Extraction, Wester Blotting, PCR, qPCR, Single Cell Preparation, siRNA Knockdown, SgRNA Knockout, RNA Sequencing, Transfection, Transduction Histology: Cryosection, Immunohistochemistry, Immunofluorescence, FISH Animal Models: Extensive experience in working with Syngeneic, Cell Derived and Patient Derived Mouse Model of Cancer including Cell/Tissue Implantation, Survival Surgery, Drug Treatment (IP/PO/IV), Blood Collection, And Tissue Harvesting, Animal MRI Imaging, Bioluminescent Imaging, Radiation Data Analyses Tools and Software: Proficient in Working with Microsoft Word, Excel, Power Point, Graphpad Prism, SPSS, Image J, Compusyn Softwre, Winlist for Flow Cytometry Analysis, Biostatistical and Other Data Analyses. Show More
Passionate about the intersection of science, leadership, and community engagement, I am a biochemistry student and researcher dedicated to advancing ... View More
Top Skills RNA interference (RNAi) & gene knockdown techniques C. elegans handling, lifespan assays, and synchronization Fluorescence microscopy & mitochondrial stress analysis Molecular biology techniques (NGM plate prep, bacterial culture) Data collection & statistical analysis (Kaplan-Meier survival curves, ANOVA) Show More
