knockdown Search ResultsExperienced scientist with a strong background in research and analysis. Proficient in experimental design, data analysis, and interpretation across v... View More
Top Skills Expertise in molecular biology techniques like plasmid isolation, genomic DNA isolation, total RNA isolation, cDNA preparation, PCR cloning, ligation, transformation, electroporation, transfection, restriction digestions, Southern blotting, and construct development for knockout and knockdown of Mtb I have experience in the development of knock-ins in nematodes using CRISPR-Cas9 Working experience in nematode gonadal microinjection for gene manipulation 1.5 years of post-graduate experience in the R&D sector of ARA Healthcare, Gurugram, on the process development of recombinant therapeutic protein Expertise in the development of recombinant strains (knockout and knockdown) of Mtb Show More
Highly skilled Postdoctoral Researcher with extensive experience in biomedical research. Recognized for innovative approaches, analytical problem-solv... View More
Top Skills Working experience in cancer biology Mammalian cell culture maintenance (adherent, suspension) including cancer and virus producing cell lines. Nucleic acid purification and Western blot analysis. Expertise in Reverse transcription quantitative PCR (RT-qPCR)- Real Time Array and Real Time PCR. Proficiency in confocal microscopy. Show More
Ph.D. Molecular biologist with 12+ years of research experience specializing in gene therapy, viral vector biology, and quantitative analytical method... View More
Top Skills Cell Biology & Assays: Mammalian and primary cell culture, stable cell line development transfection (lipid/electroporation), stable cell line development, assay development & optimization, lentiviral/AAV transduction & titration Molecular Biology: CRISPR/Cas9 gene editing, RT-qPCR, RNA/DNA extraction, cloning, siRNA knockdown, ELISA, Western blot, proteomics (SILAC, LC-MS/MS) Imaging & Cytometry: Confocal microscopy, ICC/IHC, flow cytometry In Vivo: Mouse models of bone marrow failure & retinal disease — handling, dosing, tissue collection & processing Bioinformatics & Data: GraphPad Prism, ImageJ, basic data analysis & visualization Show More
Dedicated Certified Journeyman Carpenter with expertise in metal framing, drywall installation, and mentoring. Committed to enhancing project efficien... View More
Top Skills Specialized in all gauges of metal framing (12 gauge to 25 gauge). Installed all types of metal and wood frame doorbucks including knockdown and solid frame. Installed all forms of drywall including densglass and plywood including T-111, durock and coreboard installation including framing. Specialized in wood and metal protection on the job site. Specialized in all forms of acoustical ceilings including drywall. Show More
Ph.D. with an extensive knowledge and expertise of Cellular & Molecular Biology and Biochemistry primarily in Breast Cancer and Immunology.
20 years o... View More
Top Skills Molecular work. High Throughput Single Cell RNA Sequencing Chromatin-Immuno Precipitation assay (ChIP), Chip-SEQ analysis, Transcription co-factors, Histone methylation RNA interference: experience with developing, validating, and applying oligonucleotide and viral (si and sh) RNAi reagents, for transcript/protein knockdown. Retro-Viral Vectors, transfection, On/Off system. Show More
Senior Scientist with 10+ years of experience in cellular and biochemical assay development, targeted protein degradation, high-throughput screening, ... View More
Top Skills Targeted Protein Degradation: HiBiT degradation assay, HaloProtac3 assay, NanoBRET ternary complex formation, TR-FRET, AlphaLISA, NanoBiT, CETSA, Target engagement assays, Cell permeability assays, HiBiT blotting, E3 ligase deconvolution (BioID, Co-IP/MS), In vitro and in vivo ubiquitination assays High-Throughput Screening & Automation: 1536-well HTS miniaturization, HighRes BioSolutions integrated automation (Cellario software), Mosquito LV liquid handler, TR-FRET and fluorophore-based biochemical HTS, Assay QC (Z', S/B, EC50), TIBCO Spotfire data analysis and visualization Cell-Based & Biochemical Assays: Show More
Graduate researcher specializing in human pluripotent stem cell biology and hematopoietic differentiation. Experienced in CRISPR-Cas9–based functional... View More
Top Skills Cell and Molecular Biology: Cell culture, gene knockdown/overexpression, qRT-PCR, and Western blot Genetic Engineering: Proficient in CRISPR-Cas9–based genome editing, inducible Cas9 systems, and pooled sgRNA library screening for functional genomics. Flow Cytometry and Imaging: FACS analysis for cell surface markers. Professional Competencies: Experimental design, data visualization, and manuscript preparation Show More
Accomplished biomedical and veterinary scientist with expertise in molecular biology, immunology, microbiology, cancer biology, bioinformatics, and da... View More
Top Skills Research & Molecular Expertise Extensive experience in molecular biology, cell biology, microbiology, immunology, and cancer biology through postdoctoral and graduate research roles. Skilled in mammalian cell culture, lentiviral transduction, gene knockdown, PCR/qPCR, protein analysis (Western blot, ELISA), and flow cytometry. Investigated host-pathogen interactions, immunopeptidomics, and biomarker discovery for cancer and infectious disease research. Laboratory Leadership & Quality Assurance Directed laboratory operations, staff, SOPs, and workflows as Microbiology Department Head. Implemented and maintained ISO 17025-compliant Quality Management Systems, including audits, documentation, and corrective actions. Certifications Data Science and Machine Learning Certificate, Massachusetts Institute of Technology (MIT), 2025. ISO/IEC 17025 Laboratory Quality Management: Internal Auditing, SOP Development, Validation, Measurement Uncertainty, Laboratory Management, Internal Assessor, Quality Assurance & Biosafety. Certificate of Recognition for Outstanding Contribution in Implementation of ISO/IEC 17025, Animal Health Institute (formerly NAHRC/NAHDIC). Show More
Passionate about the intersection of science, leadership, and community engagement, I am a biochemistry student and researcher dedicated to advancing ... View More
Top Skills RNA interference (RNAi) & gene knockdown techniques C. elegans handling, lifespan assays, and synchronization Fluorescence microscopy & mitochondrial stress analysis Molecular biology techniques (NGM plate prep, bacterial culture) Data collection & statistical analysis (Kaplan-Meier survival curves, ANOVA) Show More
Experienced research professional with a strong academic background from the University of Chicago, specializing in immune-oncology. Proficient in mol... View More
Top Skills Molecular Biology: Genome-wide CRISPR screen and NGS analysis Generating knockouts, knockdowns (stable or inducible), overexpression vectors Proficient in basic molecular biology techniques: qPCR, PCR, Western blot, DNA/RNA isolation, scRNA-sequence Cell Culture: Creating knockout or knockdown cell lines (stable or inducible) Culturing human or mouse primary cells and cell lines Handling mouse ES cells Lentivirus transduction Car-T cell culture Primary T cell culture and stimulation Recombinant protein expression in cell line Generating reporter cell lines Immunology: Flow cytometry analysis ELLISPOT assay ELISA (enzyme-linked immunosorbent assay) Cell sorting techniques Tumor Models: Designing, developing, and validating therapeutic candidates in tumor models Generating tumor immunology mouse models Designing and creating knock-in and knock-out mouse models Conducting tumor preclinical experiments using GEM (genetically engineered mouse) tumor models and transplantation models Different dose of drug administration in tumor models (IP, IV, Intratumoral) Other Skills: Designing lipid synthesis experiments and performing mass spectrometry analysis Immunofluorescence (IF) staining of human tissues and cell lines Confocal imaging Ferroptosis detection assays Drug screening using reporter cell lines Writing and publishing scientific articles Presenting data in meetings Excellent communication skills Show More
