Summary
Overview
Work History
Education
Skills
Publications
Trainings/Workshops Attended
Conference Attended
References
Timeline
BusinessAnalyst
Dr. Vaibhav Gujar

Dr. Vaibhav Gujar

Senior HPLC Analyst
Nagpur,MH

Summary

  • More than 10 years of experience in Biotechnology, Genomics, Bioinformatics, Proteomics, Enzymes, Fermentation and Applied Microbiology.
  • 9 years of experience in HPLC/UHPLC Method Development & Validation, HPLC/UHPLC troubleshooting and Maintenance. Expertise in developing creative methods to investigate complex analytes like dyes, pigments & lake substances.
  • Familiar with Software related to Spectroscopic Techniques like BioRad-KnowItAll, Analysis of IR, NMR, MS, UV spectras on KnowItAll software. FTIR Spectra analysis using FDM, ST-JAPAN, Agilent libraries.
  • 3 years of experience in GLP Compliance, preparation of SOPs & Study Plan according to GLP Regulations. Experience in EcoTox studies in OECD & REACH Regulations. Expertise in Substance Identification.
  • Worked on project on Enzymatic synthesis of fructo-oligosaccharides (FOS), analysis of oligosaccharides on HPLC-RID & enzyme characterization.
  • Publications on Fungal Genomics, enzyme structure prediction and prediction of active site residues in enzymes using bioinformatics tools. Bioinformatics analysis of CAZymes in fungal genome, structure prediction, prediction of active sites and binding site in CAZymes.
  • Handled a 10 litre fermenter for production of fructosyltransferase from Aspergillus oryzae, and synthesis of FOS in a batch reactor while working as Junior Research Fellow (JRF) in CSIR-CFTRI.
  • Taught undergraduate students the disciplines of Biotechnology and Microbiology, created lesson and curriculum plans. Coached students on standard laboratory techniques.

Overview

19
19
years of post-secondary education
10
10
years of professional experience

Work History

Senior HPLC Analyst

GPC Regulatory India Private Limited
Nagpur, Maharashtra
04.2018 - Current
  • Working as Senior HPLC Analyst in GPC Regulatory India Pvt. Ltd. (NS Support Services Pvt. Ltd.); mainly involved in method development and validation of industrial chemicals using HPLC-DAD and HPLC-RID. Actively involved in the maintenance & troubleshooting of HPLC. Till now analyzed more than 400 registered chemicals (for ECHA) under REACH Regulation. Expertise in developing strategies for analysis of test chemical in EcoTox studies.

Senior Research Fellow

CSIR-NEERI
Nagpur, Maharashtra
05.2014 - 02.2018
  • Worked as Senior Research Fellow in CSIR-NEERI, Nagpur, Maharashtra, India on Project “Production of Fructo-oligosaccharides using fungal fructosyltransferase”.

Junior Research Fellow

CSIR-CFTRI
Nagpur, Karnataka
08.2012 - 04.2014
  • Worked as Junior Resarch Fellow in CSIR-CFTRI, Mysore, Karnataka, India on Project “Biotechnological production of Inulo-oligosaccharides by inulinase from Aspergillus oryzae”.

Lecturer

UG Department of Biotechnology, Shri Shivaji Science College
Nagpur, Maharashtra
07.2010 - 05.2011
  • Evaluated and revised lesson plans and course content to facilitate and moderate classroom discussions and student-centered learning in Biotechnology theory and practicals.
  • Supported graduate students to participate in National Conference in Biotechnology and present poster on applications of biotechnology.

Lecturer

UG Department of Biotechnology, Shri Brijlal Biyani Science College
Amravati, Maharashta
08.2009 - 05.2010
  • Delivered lectures to graduate students in Biotechnology subject.
  • Inspired discussions through presentation of thought-provoking material, preparing students for rigors of Biotechnology field.

Education

Ph.D. - Biological Sciences

AcSIR, CSIR-NEERI, Nagpur
Nagpur, Maharashtra
05.2014 - 02.2018

Master of Science - Biotechnology

SGB, Amravati University
Amravati, Maharashtra
06.2006 - 07.2018

Bachelor of Science - Microbiology

SGB Amravati University
Amravati, Maharashtra, India
06.2003 - 07.2006

Skills

    Food Analysis: Pathogens, Allergens, Toxins, Pesticide Residues, Antibiotic Residues testing

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Publications

1. Gujar VV, Khardenavis AA, Purohit HJ, (2017) Recent trends in bioprocess optimization of fructo-oligosaccharides. In Rai V, Kumar P, (Eds.) Recent Advances in Biotechnology, Vol. 3, Shri Publishers and Distributers, New Delhi, pp 215-238.

2. Fuke P, Gujar VV, Khardenavis AA, Purohit HJ (2017) Genome Annotation and Validation of Keratin-Hydrolyzing Proteolytic Enzymes from Serratia marcescens EGD-HP20. Appl Biochem Biotechnol. doi: 10.1007/s12010-017-2595-0

3. Gujar VV, Fuke P, Khardenavis AA, Purohit HJ (2018) Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from Penicillium chrysogenum Strain HKF42. Indian J Microbiol doi: 10.1007/s12088-017-0704-y

4. Gujar VV, Fuke P, Khardenavis AA, Purohit HJ (2018) Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes. 3 Biotech 8, 106. doi:10.1007/s13205-018-1132-3

5. Fuke P, Gujar VV, Khardenavis AA, Purohit HJ (2021) Process development for keratinous waste valorisation using Serratia marcescens EGD-HP20 (submitted)

6. Gujar VV., Khardenavis AA., Purohit HJ (2021) Optimization of culture medium for enhanced production of β-fructofuranosidase by Response Surface Methodology and biochemical characterization (To be submitted)

7. Chavan AR, Nakhate SP, Singh AK, Gupta RK, Poddar BJ, Gujar VV, Purohit HJ,Khardenavis AA (2021) Recent trends in the biotechnology of functionalnon-digestible oligosaccharides with prebiotic potential (Submitted)

Trainings/Workshops Attended

  • International Workshop on “Advances in Disinfection Technologies” held at National Environmental Engineering Research institute, Nagpur on 9th February 2011.
  • National workshop cum training Programme on “Computational Approaches to Phylogenetic Analysis” organized at Bioinformatics Infrastructure Facility, Dept of Biotechnology, SGB Amravati University Amravati during 26-28th March 2012.
  • Short term training program on “Computational Drug Design using Molecular Docking and Virtual Screening” held at Department of Chemistry, Visvesvaraya National Institute of Technology (VNIT), Nagpur on 12-14th March 2015.
  • The training programme on “Chromatographic Based Analytical Techniques to Ensure Food Quality & Safety Issues” held at CSIR-CFTRI, Mysore during Sep 24-28, 2018.
  • Virtual Remote Online Training Program on “Concept of Hazardous andRestricted Chemicals Testing” Organized by National Accreditation Board forTesting and Calibration Laboratories (NABL), held on 04, August 2020

Conference Attended

  • 57th Annual Conference of association of Microbiologist of India & International Symposium on “Microbes and Biosphere: What’s New What’s Next” on November 24-27, 2016, Gauhati University, Guwahati, Assam, India. Poster presented on “Fermentative production of Fungal Fructosyltransferase”.
  • Symposium on “Interdisciplinary approaches Towards Environment Management” on September 22nd, 2017 organized by CSIR-NEERI, Nagpur.
  • International Conference on Emerging Trends in Biotechnology for Waste Conversion (ETBWC), 2017 XIV Annual Convention of The Biotech Research Society India (BRSI), on October 8-10, 2017 Organized By CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur Maharashtra. Poster presented on “Optimization of culture medium for enhanced production of β-fructofuranosidase by Response Surface Methodology”

References

1. Dr. Anshuman A. Khardenavis, Principal Scientist

Environmental Biotechnology and Genomics Division

CSIR-NEERI, Nehru Marg, Nagpur

Email: aa_khardenavis@neeri.res.in

Mobile: +91 8605596552

2. Dr. Hemant J. Purohit, Retired Chief Scientist & Head

Environmental Biotechnology and Genomics Division

CSIR-NEERI, Nehru Marg, Nagpur

Email: hemantdrd@hotmail.com

Mobile: +91-9850397205

3. Dr. Anita S. Patil, Professor,

Department of Biotechnology,

Sant Gadge Baba, Amravati University,

Amravati- 444602

Email: patilas12@yahoo.co.in,

anitapatil@sgbau.ac.in

Mobile: +919881735354

Timeline

Senior HPLC Analyst

GPC Regulatory India Private Limited
04.2018 - Current

Senior Research Fellow

CSIR-NEERI
05.2014 - 02.2018

Ph.D. - Biological Sciences

AcSIR, CSIR-NEERI, Nagpur
05.2014 - 02.2018

Junior Research Fellow

CSIR-CFTRI
08.2012 - 04.2014

Lecturer

UG Department of Biotechnology, Shri Shivaji Science College
07.2010 - 05.2011

Lecturer

UG Department of Biotechnology, Shri Brijlal Biyani Science College
08.2009 - 05.2010

Master of Science - Biotechnology

SGB, Amravati University
06.2006 - 07.2018

Bachelor of Science - Microbiology

SGB Amravati University
06.2003 - 07.2006
Dr. Vaibhav GujarSenior HPLC Analyst