Animal Reproduction and Biotechnology Laboratory (ARBL)
Institute of Animal Physiology and Genetics
Department of Animal Science
- Animal Physiology
- Animal Reproduction
- Animal Genetics
- Applications of Biotechnology in Animal Production.
- Scientific Thinking and Writing.
Institute of Animal Sciences
Department of Animal Science
My research interests primarily revolve around the domain of animal reproductive physiology, specifically within the field of embryology My particular emphasis lies in investigating extracellular vesicles (EVs) and their molecular cargos (specifically miRNAs) in modulating embryonic development and cellular stress responses
2012-2017: Co-supervisor of embryology and cell culture research lab, Cairo University Research Park (CURP).
2015-2017: Deputy Director for International Agreements, International Relations Office, Cairo University (IRO-CU).
2013-2017: Coordinator of International Relations Office- Faculty of Agriculture, Cairo University (IRO-Agr).
2013-2015: Coordinator of Scientific Research at the Department of Animal Science, Cairo University.
-2024: Research/Travel grant of the postdoctoral association. Colorado State University, CO
-2024: 2nd position award, Annual Postdoc Research Symposium. Colorado State University, CO
-2016: National incentive award for Advanced Technological Sciences that Serve the Agricultural Fields. Ministry of Scientific Research, Egypt.
-2016: University incentive award in Agricultural Sciences. Cairo University, Egypt.
-2012: 1st prize for the best scientific poster at the 16th Conference of the Egyptian Society of Animal Production (ESAP). Cairo, Egypt
-2009: 1st prize of the student competition in the 25th Scientific Meeting of the Association of Embryo Technology in Europe (AETE), Poznan, Poland.
-2008: DAAD Ph.D. Fellowship. DAAD, Germany
-2007: The best scientific Master Thesis in the field of Animal Production for the academic year 2006/2007. Cairo University, Egypt.
-2006: 1st prize for the best scientific poster in the 13th Conference of the Egyptian Society of Animal Production (ESAP), Cairo, Egypt.
-2006: 2nd prize in the annual competition of The Best Scientific Poster for the Young Scientist. Faculty of Agriculture, Cairo University, Cairo, Egypt.
2024-Current: Postdoc Teaching Certificate of Completion Program, CSU, Fort Collins, CO
1) M.Sc. Thesis: Kateřina Marcollová (2022): The role of microRNAs in regulation of mammalian oocyte and embryo development. Institute of Animal Physiology and Genetics, Czech Academy of Science, Libechov, Czech Republic.
2) Ph.D. Thesis: Dalia Ahmed (2020): Effect of Stress Factors on Bovine Embryonic Development. Department of Animal Production, Faculty of Agriculture, Cairo University.
3) M.Sc. Thesis: Mohamed Magdy (2019): In vitro production and vitrification of buffalo embryos. Department of Animal Production, Faculty of Agriculture, Cairo University.
4) M.Sc. Thesis: Said Said (2019): Molecular Analysis of Oocyte Maturation in Egyptian Buffalo Using Different Media Supplements. Department of Genetics, Faculty of Agriculture, Cairo University.
5) M.Sc. Thesis: Rasha Hassan (2019): Experimental Study of Using DNA in Analyzing and Dating Papyrus Manuscripts & Evaluating some Methods of Consolidation, With Application on a Selected Object. Faculty of Archaeology, Cairo University.
6) Ph.D. Thesis: Aiah El-Rashidy (2017): Evaluation of Antibacterial Activity of Zein Scaffolds Containing Silver-Doped Bioactive Glass Versus Silver-Free Scaffolds: An In Vitro Study. Biomaterials Department, Faculty of Oral and Dental Medicine, Cairo University.
7) M.Sc. Thesis: Aiah El-Rashidy (2014): Chemical and Biological evaluation of type (I) Collagen extracted by two techniques from fish scales for dental use. Biomaterials Department, Faculty of Oral and Dental Medicine, Cairo University.
a) Association of Embryo Technology in Europe (AETE), France, since 2013
b) International Embryo Technology Society (IETS), USA, since 2011
c) Egyptian Society of Animal Production (ESAP), Egypt, since 2001
a) Frontiers in Veterinary Science- Animal Reproduction ( Guest Associate Editor )
b) Frontiers in Cell and Developmental Biology ( Review Editor )
c) Frontiers in Physiology. ( Review Editor )
d) Cells ( Topic Editor )
e) Journal of Animal Reproduction and Biotechnology
f) World Journal of Veterinary Science
g) Research in Agriculture, Livestock and Fisheries
Scopus:
- H-index: 17
- Documents: 47
- Total citations: 1161
Web of Science:
- H-index: 18
- Documents: 51
- Total citations: 1061
- Verified review activity: 85 peer review records in 32 scientific journals.
- Verified editor records: 2
-2024-
1) Joyce K., Gad A., Menjivar N.G., Gebremedhn S., Heredia D., Dubeux G., Lopez-Duarte M.C., Bittar J., Gonella-Diaza A, Tesfaye D. (2024)
Seasonal environmental fluctuations alter the transcriptome dynamics of oocytes and granulosa cells in beef cows. J Ovarian Res 17: 201.
2) Feugang J; Gad A ; Menjivar N.G; Ishak G.M; Gebremedhn S; Gastal M.O; Dlamini N.H; Prochazka R; Gastal E.L; Tesfaye D. (2024) SeasonalInfluence on miRNA Expression Dynamics of Extracellular Vesicles in Equine Follicular Fluid. J. of Anim. Sci. and Biotech. 15:137
3) Gad A , Menjivar N.G, Felton R, Durrant B, Tesfaye D, Ruggeri E (2024) Mapping the follicle-specific regulation of extracellular vesicle-mediatedtransport in the southern white rhinoceros (Ceratotherium simum simum) Biology of Reproduction 111 (2): ioae08.
4) Kennedy V.C, Lynch C.S, Tanner A, Winger Q.A, Gad A , Rozance P.J and Anthony R.V (2024) Fetal Hypoglycemia Induced by Placental SLC2A3RNA Interference Alters Fetal Pancreas Development and Transcriptome at Mid-Gestation. Int. J. Mol. Sci. 25 (9), 4780.
5) Bartkova A, Nemcova L, Strejcek F, Gad A , Kinterova V, Morovic M, Benc M, Prochazka R, Laurincik J (2024) Impact of media supplementsFGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro. Sci Rep 14, 7081
6) Menjivar N.G, Oropallo J, Gebremedhn S, Souza L.A, Gad A , Puttlitz C.M, Tesfaye D (2024) MicroRNA nano-shuttles: Engineering extracellularvesicles as a cutting-edge biotechnology platform for clinical use in therapeutics (2024) . Biological Procedures Online 26 (14)
-2023-
7) Menjivar N, Gad A , Thompson R, Meyers M, Hollinshead F, Tesfaye D (2023) Bovine Oviductal Organoids: A multi-omics approach to capturethe cellular and extracellular molecular response of the oviduct to heat stress. BMC Genomics 24:646
8) Gad A , Joyce K, Menjivar N, Heredia D, Rojas C, Tesfaye D, Gonella-Diaza A (2023) . Extracellular vesicle-microRNAs mediated response ofbovine ovaries to environmental heat stress. J Ovarian Res 16:101
9) Sakr OG, Gad A , Cañón-Beltrán K, Cajas YN, Prochazka R, Rizos D, Rebollar PG. (2023) . Characterization and identification of extracellularvesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks. Theriogenology 209: 76-88
10) Menjivar N, Gad A , Ghosh S, Tesfaye D (2023) . Granulosa cell-derived extracellular vesicles mitigate the detrimental impact of thermal stresson bovine oocytes and embryos. Frontiers in Cell and Developmental Biology , 11: 1142629
11) Gebremedhn S, Gad A , Ishak G, Menjivar N, Gastal M, Feugang J, Prochazka R, Tesfaye T, Gastal E (2023). Dynamics of extracellular vesicle coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation. Molecular Human Reproduction 29 (4)
12) Murin M, Nemcova L, Bartkova A, Gad A , Lucas-Hahn A, Strejcek F, Prochazka R, Laurincik J (2023) Porcine oocytes matured in a chemicallydefined medium are transcriptionally active. Theriogenology 203:89-98
13) Dlamini N, Nguyen T, Gad A , Tesfaye D, Liao S, Willard S, Ryan P, and Feugang J (2023) Characterization of Extracellular Vesicle-CoupledmiRNA Profiles in Seminal Plasma of Boars with Divergent Semen Quality Status. Int. J. Mol. Sci. 24(4), 3194
-2022-
14) Gad A ; Murin M; Bartkova A; Kinterova V; Marcollova K; Laurincik J; Prochazka R (2022) Small-extracellular vesicles and their microRNA cargofrom porcine follicular fluids: the potential association with oocyte quality. J. of Anim. Sci. and Biotech. 13:82
- 2021 -
15) Procházka R, Bartková A, Němcová L, Murín M, Gad A , Marcollová K, Kinterová V and Laurinčík J (2021) The role of MAPK3/1 and AKT in the , acquisition of high meiotic and developmental competence of porcine oocytes cultured in vitro in FLI medium. International Journal ofMolecular Sciences 22: 11148
16) Faheem M, Ghanem N, Gad A , Procházka R, Dessouki S (2021) Adaptive and biological responses of buffalo granulosa cells exposed to heatstress under in vitro condition. Animals 11 (3): 794.
17) Ghanem N, Ahmed D, Desouki S, Faheem M, Gad A, Peippo J, Barkawi A (2021) : Cellular and molecular alterations of buffalo oocytes culturedunder two different levels of oxygen tension during in vitro maturation. Zygote, 1-11 .
18) Saadeldin I, Gad A and Mermillod P (2021) Editorial: Biofluid Extracellular Vesicles and Their Involvement in Animal Reproductive Physiology. Front. Vet. Sci 8:747138
19) Benc M, Strejcek F, Morovic M, Bartkova A, Murin M, Gad A , Bonnet-Garnier A, Popovska-Percinic F, Laurincik J (2021) Improving the qualityof oocytes with the help of nucleolotransfer therapy. Pharmaceuticals 14(4), 328
-2020-
20) Gad A, Murín M, Nemcova L, Bartkova A; Laurincik J, Procházka R (2020) : Inhibition of miR-152 during in vitro maturation enhances thedevelopmental potential of porcine embryos. Animals 10 (12): 2289. IF: 2.75
21) Gad A , Sánchez JM, Browne J, Nemcova L, Laurincik J, Prochazka R, Lonergan P (2020) Plasma extracellular vesicle miRNAs as potentialbiomarkers of superstimulatory response in cattle. Scientific Reports 10: 19130, 1-16. IF: 4.38
22) Gebremedhn S, Gad A, Aglan H, Laurincik J, Prochazka R, Salilew-Wondim D, Hoelker M, Schellander K, Tesfaye D (2020) Extracellularvesicles shuttle protective messages against heat stress in bovine granulosa cells. Scientific Reports 10: 15824, 1-19. IF: 4.38
23) Koncicka M, Cervenka J, Jahn D, Sucha R, Vodicka P, Gad A, Alsheimer M, Susor A (2020) Expression of lamin C2 in mammalian oocytes. PlosOne 15(4):e0229781. doi.org/10.1371/journal.pone.0229781. IF: 3.24
24) Gad A., Nemcova L., Murin M., Kinterova V., Kanka J., Laurincik J., Benc M., Pendovski L., Prochazka R. (2020) Global transcriptome analysisof porcine oocytes in correlation with follicle size. Molecular Reproduction and Development 87 (1): 102-114 doi:10.1002/mrd.23294. IF: 2.609
25) Ahmed D, Ghanem N, Dessouki S, Faheem M, Gad A and Barkawi A (2020) Developmental Competence of Buffalo Oocytes Cultured UnderDifferent Oxygen Tensions after Selection with Brilliant Cresyl Blue. World's Veterinary Journal 10 (2): 256-263
26) Ashour G, Gad A , Fayed A.K, Ashmawy N, El- Sayed A (2020): Evaluation of growth performance, blood metabolites and gene expressionanalysis in Egyptian sheep breeds in relation to age. World's Veterinary Journal 10 (1): 18-29
27) Gebremedhn S, Ali A, Gad A , Procházka R, Tesfaye D (2020): Extracellular Vesicles as Mediators of Environmental and Metabolic stress CopingMechanisms during Mammalian Follicular Development. Front. Vet. Sci. 7: 961
-2019-
28 ) Gad A., Nemcova L., Murin M., Kanka J., Laurincik J., Benc M., Pendovski L., Prochazka R. (2019) MicroRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles. Molecular Reproduction and Development 86 (4):426–439 .
29 ) Sakr O., Gad A. , Rodríguez M., Rebollar P., Millánc P. (2019) Superoxide dismutase mimics improve semen quality during chilled preservation of rabbit spermatozoa. Livestock Science. 221: 70-76
For the full list please visit the following link: https://vetmedbiosci.colostate.edu/directory/member/?id=42109
2024- Oral presentation: Annual Postdoc Research Symposium, Colorado State University, Fort Collins, CO
2024- Poster: 50th Annual Conference of the International Embryo Technology Society (IETS), Denver, CO
2023- Invited Speaker: Institute of Animal Physiology and Genetics Conference, Libechov, Czech Republic
2023- Poster: 16th annual Rocky Mountain Reproductive Sciences Symposium, Fort Collins, CO
2022- Poster: 55th Society for the Study of Reproduction (SSR) Annual Meeting, Online
2022- Oral presentation: 15th annual Rocky Mountain Reproductive Sciences Symposium, Fort Collins, CO
202- Invited Speaker: IPB University, Indonesia, Online Summer Course Program, Online
2021- Poster: 37th Scientific Meeting of the Association of Embryo Technology in Europe (AETE), Online
2021- Poster: 2nd Conference of the Visegrád Group Society for Developmental Biology, Szeged, Hungary
2020- Poster: 36th Scientific Meeting of the Association of Embryo Technology in Europe (AETE-Lite), Online
2019- Poster: 35th Scientific Meeting of the Association of Embryo Technology in Europe (AETE), Murcia, Spain
2018- Poster: 22nd Annual ESDAR Conference, Cordoba, Spain
2018- Oral presentation: Visegrád Group Society for Developmental Biology Inaugural Meeting, Brno, Czech Republic
2017- Poster: 43rd Annual Conference of the International Embryo Technology Society (IETS), Austin, TX
2016- Invited Speaker: 17th Conference of the Egyptian Society of Animal Production (ESAP), Sharm El-Sheikh, Egypt
2016- Poster: 42nd Annual Conference of the International Embryo Transfer Society (IETS), Louisville, KY
2015- Poster: 41st Annual Conference of the International Embryo Transfer Society (IETS), Versailles, France
2013- Invited Speaker: 4th Mammalian Embryo Genomics meeting, Quebec City, Canada
2013- Poster: 29th Scientific Meeting of the Association of Embryo Technology in Europe (AETE), Istanbul, Turkey
2013- Poster: 39th Annual Conference of the International Embryo Transfer Society (IETS), Hannover, Germany
2012- Invited Speaker: 16th Conference of the Egyptian Society of Animal Production (ESAP), Cairo, Egypt
2011- Invited Speaker: 3rd Embryo Genomics Meeting, Bonn, Germany
2011- Oral presentation: Deutschen Gesellschaft für Züchtungskunde (DGFZ), Freising, Germany
2011- Oral presentation: 38th Jahrestagung der Arbeitsgemeinschaft Embryotransfer deutschsprachiger Länder (AET-d), Kleve, Germany
2010- Oral presentation: Deutschen Gesellschaft für Züchtungskunde (DGFZ), Kiel, Germany
2010- Oral presentation: 37th Jahrestagung der Arbeitsgemeinschaft Embryotransfer deutschsprachiger Länder (AET-d), Bonn, Germany
2009- Oral presentation: 25th Scientific Meeting of the European Embryo Transfer Association (AETE), Poznan, Poland
2007- Poster: 58th Annual Meeting of the European Association for Animal Production (EAAP), Dublin, Ireland
2006- Poster: 13th conference of the Egyptian Society of Animal Production (ESAP), Cairo, Egypt
2024-Current: Postdoc Teaching Certificate of Completion Program, CSU, Fort Collins, CO
Erasmus+: Internationalization of Universities, University of Porto, Porto, Portugal
Sustainable Agro-Ecosystem Management, University of Sassari, Sassari, Italy
Next-Generation Sequencing: Whole Genome Sequencing Library Preparation, EMBL, Heidelberg, Germany
Perspectives on Science & Technology Diplomacy for Sustainable Development in NAM and Other Developing Countries, NAM S&T Center, Haryana, India
Principles of Good Teaching in Higher Education, DAAD Kairo Akademie, Cairo, Egypt
R program and its Applications in Statistics, University of Bonn, Bonn, Germany
Bioinformatics for Animal and Plant Scientists, Humboldt Universität, Berlin, Germany
MiRNA Detection and Analysis, QIAGEN & EMBL, Heidelberg, Germany
Microarray Data Analysis, AIRBB, Torino, Italy